In article <53e4qc$pgp at net.bio.net> Laura A. Cox, lCOX at icarus.sfbr.org
writes:
> 1.Can anyone tell me precisely how to pour the plates without tape?
>> I watched someone do it with some 34cm wtr plates but when I tried
>> to do it with some 48cm wtr plates it was a disaster. (snipped)
As well as the method above (pippetting a bead of gel onto the lower
plate, and
lowering or sliding the upper plate on), you can also just clamp the
plates.
We now have a 377 with its cassette, but before that I was pouring 0.4mm
gels
horizontally by clamping the plates together with about 6 bulldog clips
(38cm
gels, I think) and then just running the gel in from a syringe. I think
the
key must be clean plates, so that the gel runs in cleanly, but doesn't
run out
of the bottom. Incidentally, I once tried hot glue to seal plates
following a
methds.rgts thread - disaster! The glue expanded as it cooled, so the
comb was
loose!
Anthony.
