Agnes Gallacher; agnes at hgu.mrc.ac.uk wrote in article
<53vh48$ma2 at net.bio.net>...
> We're interested in trying out some ready-made gels and wondered if
> anyone can recommend any. If you've tried any were you successful
> Agnes.
>>
We have been using Sequagel 4 and 6 (for 4% and 6% gels) for about 6
months
now and have found them very good. They are available from National
Diagnostics. Sequagel only requires the addition of 10% APS, does not
need
de-gassing and sets in about 10 minutes after pouring.
Although red rain does not seem to be a problem, PCR products over about
250 bp so seem to get a bit smeary for genotyping if the gel has been left
to set overnight.
Hope this helps.
Ed Ryder
The Animal Health Trust
PO Box 5
Newmarket
England
mail: eryder at hgmp.mrc.ac.ukTed at roswell.dungeon.com