In article <52voin$g6d at net.bio.net>, Janice Wahlstrom,
janice_wahlstrom at qm.salk.edu wrote:
> We are in the process of setting up an automated DNA sequencing core
> facility here at the Salk Institute. We will be starting out with 2 ABI
> 377 machines...On a good day, with 48cm
> plates we can get about 700 good bases maximum. Often the reads are
> significantly shorter. Therefore, one issue is how to modify the
> protocol to maximize read length. Any suggestions for changes (maybe
> voltage, acrylamide %, running temp, buffer)?
This may not help you much if you have already purchased your sequencers,
but in our core lab we have an ABI377 AND a Li-Cor 2000L. Each fills a
niche, we run both daily. Having both solves the problem of how to have
high throughput capacity and also provide long reads when required. The
Li-Cor, running 66cm Long Ranger gels, averages about 900 bases, and some
of our users can routinely get 1200 base reads.
Lawrence Washington
Indiana Molecular Biology Institute
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Lawrence Washington
Indiana Molecular Biology Institute
Bloomington, Indiana
