Hi
Thanks to all those who responded to my question, here are snipets from
each response.
In short (for those who don't want to read further)
It would appear that -20 is ideal for storage of dried pellets for upto 3
months (at least), not that anyone every has samples sitting around that
long.... "These samples are very Important!!!"
>>I have stored dried down cycle sequenced rxns in the refrigerator for
>>a month. I also have held them at room temp for a few days.
>>ABI supplies Taq standards which sit around for months at -20 and seem to
>>work fine. I have left them on the bench for more than a week at room temp
>>and exposed to light and they worked fine. So long as they are dry, they
>>should last a fair time at -20. I think the -70 is overkill
>>dry pellets at -20 are useable for at least a month after this although
>>longer
>>storage does produce a decline in signal strength.
>>We store our reactions at -20, and I know they are fine for a couple of
>>>>months.
>>The guys frm ABI told me that dried down reactions after EtOH ppt can be
>>stored at RT for many months, AS LONG AS THEY ARE KEPT IN THE DARK...
>>We store our Dye Terminator reaction after they are dry at -20. We
>>also store the sample+loading buffer(377 samples) at -20 in case we
>>need to rerun the sample. Can't do that with the 373 since you load
>>everything. This seems to work well. Usually our samples go on the
>>gell within 48 hr. of the time they are run so I dont know about long
>>term storage. I have used the sample+loading buffer after several
>>days when we were testing new gel formulations. They were OK.
>>I did do an experiment some years ago where I stored reactions for
>>3 weeks at -20, 4 and room temp. The -20 was just fine. The 4 gave
>>almost as good data and the room temp. still gave data but not as good.
>>Typically, we store dried-down reactions for less than a week at -20
>>degrees. However, we've had reactions stay at that temp for over 3 months
>>with no loss of signal. We also had one of those "unofficial" experiments
>>where samples sat at room temp for 3 days (in the dark...long weekend). I
>>think the most important thing is that the STAY dried-down and should be
>>kept away from long exposures with ambient light (the dyes reduce).
>From ABI
>>dried samples at -20C they are pretty stable about 1 year...the
>>sequencing standards that we supply are stored at -20C...if in formamide:
>>don't really have any data, but I've reloaded samples a couple of weeks old
>>stored at -20 with good success.
Ian
Ian Ridgers
Dept. of Zoology
The Natural History Museum
Cromwell Rd.
London. SW7 5BD
E-mail ir at nhm.ac.uk
Tel. 0171 938 9297
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