We're running a ABI 373. We use SequaGel-6 for our gels. Periodically,
we have a problem with green haze running in front of the samples. We
tried stripping plates, new gel solution (including new lot #), making our
own gel, using and not using alcohol to dry the plates, new running
buffer, soaking casting comb and comb. I think that's about it and
not one thing from the above seems to be the culprit. Any ideas,
suggestions. I should note that the haze is NOT sample or lane specific.
We have had haze where there has been no samples. ABI has no suggestions.
Thanks in advance
Susan A Baumes
sb328 at columbia.eduhttp://www.columbia.edu/~sb328