The following letter is addressed to UK users of the ABI PRISMt 377 DNA
Sequencer only, and will be sent out by mail shortly.
This does not apply to any other region not covered by the UK service group.
Dear ABI PRISMt 377 DNA Sequencer User
It has come to our attention that some customers have experienced problems with
the temperature control systems on the ABI PRISM 377 DNA Sequencer. In most
cases, these faults appear to be due to differences in the quality of tap water
and are, therefore, regional in nature.
These have ranged from water pipes blowing off to intermittent temperature
faults and leaking pushfit connectors. Any of the following symptoms should be
reported to PE Applied Biosystems Service immediately:-
d System cannot maintain a constant temperature during a run.
d A reduction in temperature followed by an over 70oC error.
d Temperature errors in the log file.
d Water leaking from underneath the instrument.
d Water leaking continuously from the front pushfit connectors.
Please note, small losses of water from the cooling system is expected due to
the fact that there is always a dead volume inside the pushfit connector which
will cause very minor leaks when removing the front heat plate. Also, the
current use of algaecide can cause a slight discolouring of water in the pipes
and/or reservoir bottle and is not, on its own, a cause for concern.
For the above, we recommend that the concentration of algaecide be changed from
Tap water containing 5 drops of Algaecide (as recommended in the User Manual)
to De-ionised water containing a 5% solution of Ethylene Glycol (Sigma E9129
or automotive anti-freeze good for aluminium blocks).
As a further value-added service, we will automatically change the coolant
within your cooling water system at future service and planned maintenance
visits. Furthermore, if the water used at installation of your ABI PRISM 377
DNA Sequencer has caused a limescale build-up inside the instrument, the
Service engineers will use a proprietary descaler and neutraliser to remove
particulates and replenish your water system with our current recommendations.
Please do not hesitate to contact me should you have any further questions
Assuring you of our best attention at all times.
Please Update your user manual as follows
Section 5. Page 14
Note : to prevent algae growth, use a 5% (v/v) solution of Ethylene Glycol (
Sigma E91929, or Automotive Anti-freeze), in High quality De-ionised water.
Section 7. Page3.
Delete: Replenishing Algecide in title and the sentence Replenishing the
algecide every month, regardless of whether you need to refill the water
Add: Top up the reservoir bottle every month with 5% (v/v) solution of Ethylene
Glycol in De-ionised water.
Section 7 page 4.
Delete: part 4... and add 5 drops of algecide ( simply add algecide if the
reservoir has sufficient water in it.)
Add: Replenish with 5% Ethylene Glycol in De-ionised water.