Automated sequencing

Christiane Robbins crobbins at nchgr.nih.gov
Wed Jul 31 07:02:46 EST 1996

HI there, I was wondering if someone has advice for the following problem:
I often see a green haze on my 377 gels ,  I can still read my data but the
gel is green. I have done everything from cleaning the plates with 3M HCL
followed with ALconox, monitoring all sets of plates to see if it's only one
set or one machine to no  avail. I still see this green haze and it's not every
gel. I would also like to know if anyone has a protocol for sequencing BAC's
with custom primers using the FS-Terminator (ABI)?
Thank you,
Christiane Robbins

Christiane Robbins
Bldg 49 Room 2C28
49 Convent Drive MSC 4431
Bethesda, MD 20892-4431

Tel# 301-402-2534
e-mail: crobbins at nchgr.nih.gov

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