We are wondering how well other people are doing in obtaining the
promised read length of 700 bp sequence with the promised accuracy of
greater than 98.5% using the 48 cm gels on the ABI 377. Is it
possible? If so what are the tricks?
We actually get better (500-550bp) than promised sequence (450)
from the short gels but when we switched to the long gels we only get
the same length of accurate (99%) sequence (500-550).
Does anyone else bother running the 48 cm gels?
All comments greatly appreciated. ABI has been of no help to us in
this.
--
Richard C. Nicholson, BSc PhD
Senior Research Fellow
Centre for Immunology
St. Vincent's Hospital
Sydney, Australia 2010 email:R.Nicholson at cfi.unsw.edu.au
