We recently tried the amplitaq gold from Perkin Elmer for
our gene mapping project. It has presented several
problems. The major one is that when compared with ordinary
taq, we got the same results but with 1/6 the peaks. Our
experiments involved fam labelled primers. We thought that
the gold would eliminate the "stutter" that is seen with the
taq. Since we followed the protocol and did obtain some
signal, albeit weaker, the amplification did work but
without the expected results. The rep has indicated that it
was probably something done incorrectly in the lab. Before
we put the time and money into improving this, is it worth
the effort? Has anyone else used this enzyme with quality
results and no stutter?
Any replies would be appreciated. Thanks in advance.
Mike
mcgorry at med.pitt.edu
--
Michael Gorry
mcgorry at med.pitt.edu
