Dear All,
Has anyone else noticed a massive drop in sequence quality, using a 48 cm
gel on an ABI 377, after the first 550 bases? Generally I get good
sequence over the first 450 - 550 bases, but past this point the peaks
become extended and indistinct. Can anyone offer any suggestions for
improving the length of readable sequence? The runs are at 2700V, 4.25%
acrylamide with dye terminating chemistry and the cycle sequencing is done
on a MJ Research DNA engine.
Any possible suggestions would be greatly appreciated,
Thanks in advance
Trish Russell
