Anybody have any experience trying to get sequence directly
from purified chromosomal DNA? We have been trying to do so with a
9.2 Mb linear chromosome without success. Our central core
sequencing facility says they just need more DNA and then they
will get sequence. Making as much as they want will be a bother,
and if sequencing directly off of a chromosome of this size is not
possible, I'd rather spend my time on some other strategy.
I should probably mention that we suspect that the
region we are trying to sequence contains a transposon.
Inverse PCR attempts to clone the region of interest gave
anomolous results, among other clues.
Thanks.
Laura Kasman
University of Georgia - Athens
Dept. of Entomology
