Ken Taylor said......
>I've seen most artefacts on 373 gels, but never red rain. But
>then I've never stripped my plates with any acids, bases or other
>noxious substances. Only soft tap water, Alconox and distilled water
>to rinse. I wonder if over zealous cleaning is what causes the gel
>to be prone to lift off the glass?
Ah! At last! Someone like me who doesn't go in for all this obsessive acid
cleaning business. I'm always reading posts to this group that say things
like 'you must HCl wash your oplates every 2 days', 'alconox clean the
buffer tanks', never use this, always do that.......
I use any old alcohol thats going to wash my plates, I only ever rinse out
the buffer tanks with normal tap water, I have cloudy 10x TBE and my gels
run perfectly, I get great reads, I get little or no gel haze. Does all
this acid cleaning just lead to more problems or am I just lucky? I dont
think its luck cos I havn't won the lottery yet :-(
Kev
Kevin Clark |
kclark at immsvr.jr2.ox.ac.uk | If you havn't seen God, you're not
Institute of Molecular Medicine| driving fast enough.
Oxford,UK. |
