A POSTDOCTORAL POSITION is currently available for large scale genomic
analysis of potato DNA homologous to the tobacco mosaic virus disease
resistance gene, N, for the purpose of isolation of potato disease
resistance genes. We have isolated the first virus resistance gene, N, of
tobacco (Whitham et al., 1994, Cell 78, 1101-1115). N shares homology with
the cytoplasmic domains of the interleukin-1 receptor and the Drosophila
Toll receptor protein. The sequence of N and several other recently
isolated resistance genes shows that they encode deduced products with
strikingly similar structural features. Their potential role as direct
receptors of pathogen encoded signals and participation in the signal
transduction pathway leading to induction of defense responses is under
investigation. Most disease resistance (R) genes are members of multigene
families. The N gene belongs to a multigene family in the highly syntenic
genomes of tobacco, tomato and potato. We have determined the location of
many N homologues in tobacco and tomato. N homologues are clustered in
several locations in these genomes, some of which are linked to known
resistance genes. We postulate that N homologues will be found linked to
resistance genes of the potato and will be useful for isolation of
resistance genes. Applicants should have experience and/or knowledge in:
molecular biology, basic recombinant DNA techniques, informatics, AFLP
analysis, large DNA cloning, sequencing and computer analysis. Prior work
with plants is not required. A cover letter describing research experience
and interests, a curriculum vitae and names of three references should be
sent to: Dr. Barbara Baker, University of California, Berkeley and USDA,
Plant Gene Expression Center, 800 Buchanan St., Albany, CA 94710. FAX:
510-559-5678; Email: bbaker at garnet.berkeley.edu
Equal Opportunity Employer.
