In article <502am2$dqj at net.bio.net> Sensational Gravity Boy <ed at bio02.bio.uottawa.ca> writes:
> We ran a gel last night (on a 373) and although the gel file looks fine,
> the samples have a spacing of -12 !!! yes, minus 12. As a result, the
> chromatograms look horrid and there are stacked bases that are
> not been called, and essentially the 30 samples we ran are a total waste.
If you still have the data file (possibly named GelFile),
try "Adaptive" or "SemiAdaptive" basecaller.
This may clear the problem of some samples.
--
ISHIKAWA Jun, Ph.D. National Institute of Health
(jun at nih.go.jp) Dept. of Bioactive Molecules
Tokyo, JAPAN
<http://www.nih.go.jp/~jun/htdocs/research/>
