Ed, you didn't say what sort of base caller you are using. We have had
similar problems to this before and found that if you attempt to analyse
using a different base caller thaen it quite often works. We have no idea
why. I hope this helps.
Sean Moore
CSIRO
Div. Food Sci. and Tech.
c/o Dept. Biochemistry
Melbourne University
Melb. Victoria, Australia
s.moore at biochemistry.unimelb.edu.au