I have been trying to use the T3 and T7 primers for sequencing
clones. The results have been terrible. When using internal
primers, I have been obtaining good reads. After analyzing
the standard T7 and T3 primers on Oligo, I have come to realize
just how bad they are. I am assuming that there are people who
use them all of the time with good results. Can anyone offer any
suggestions on how to improve the reads? I am using the ABI taq-FS
terminator kits. What should be done to optimize the results?
Thanks in advance for any help.
Mike
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Michael Gorry
mcgorry at med.pitt.edu
