Dear fellow autosequencers,
I run a LI-COR 4000L and have recently come across a
cluster of band compressions in a clone, that could not be
resolved despite the use of deaza-G and running gel at 50 C.
My questions are : is anyone using formamide-containing
gels in automated sequencing and is, say, 20-30% formamide
in the gel compatible with all these fluorescent dyes,
particularly in ABI machines? I am interested in modifying
the gel since in my case the polymerase has no problem
reading through problematic region, so that there is a true
compression, not a BAFL.
--
Alexander Kraev, PhD Internet:
kraev at bc.biol.ethz.ch
> Lab.of Biochemistry III Phone: 0041-1-632-31-47
> Swiss Federal Inst. Of Technology FAX: 0041-1-632-12-13
> Universitaetsstr. 16 Home Page: http://www.bc.biol.ethz.ch/BiochemistryIII/> CH-8092 Zurich /Sasha/kraev.html
