>Do you have opinions to share on the best choice(s) of
>software for small-scale autosequencing? We have recently acquired
>Sequencher, Phrap/Phred/Consed/etal, and Staden package,
>all of which can handle ABI and SCF trace data, do base calling
>and assembly. But it will take some time to compare and evaluate
>these, so your experiences would be valuable to us and others
>starting with such software.
>
>Thanks, Don
>
>--
>-- d.gilbert--biocomputing--indiana
u--bloomington--gilbertd at bio.indiana.edu
Don - I am only familiar with Sequencher, but as a member of the ABRF
DNA Sequencing Committee, we used Sequencher to compare and analyze 73
DNA chromatograms from ABI, ALF and Licor - and it was quite easy to
use and handled the large load gracefully. Hope this helps. Scottie
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Scottie Adams aka Pamela Scott Adams
Molecular Biology Core Facility
W. Alton Jones Cell Science Center
10 Old Barn Road
Lake Placid, NY 12946
Phone: 518-523-1276
Fax: 518-523-1849
Email: mbcf at northnet.org