> > First, on our 373, since switching to the longer 48cm plates, we have
> > had
> > intermittent problems with stoppages in electrophoresis. Some of these
> > seem to be due to leakage behind the plates, resulting in a lower top
> > buffer level and an increased bottom buffer level
Re. the leaking gels. We top the ears of the gel with agarose
gel,after the gel has polymerised, this prevents any buffer going down
the side of the spacers.Since then we have seen no leaking problems.
RE>> problem2: An ABI rep attributed Flourescence to warpped glass
plates. Are these plates old? Try another set and see if this solves
the problem.