SSCP Protocol, fluorescein

Stacia Hoover slhoover at ucdavis.edu
Sat Apr 13 03:37:15 EST 1996

Hi Sequencers,
  I was wondering if any one knew where I could find protocols to do SSCP
(single strand conformational polymorphism) analysis on the 377 sequencer.
I'm interested in gel recipes, run times, run temperature...
   Also, has any one tried to run fluorescein labelled products with the
Genescan application.  They certainly would be an easier and cheaper
alternative to the ABI dyes.


Stacia Hoover
DBS Automated DNA Sequencing
5350A Storer Hall
U.C. Davis, CA 95616
(916) 754-9259

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