>> Could someone suggest how to handle the following in a .ace file?
>> I have a cDNA that overlaps an exon-intron boundary of a genomic
> sequence, and wish to encode the cDNA as a Subsequence of the
> genomic.
>> How do I handle the cDNA coordinates to get proper graphic display
> of the sequences?
>>>> Example:
>> Sequence : "Genomic_Seq"
> DNA "Genomic_Seq" 1000
> Subsequence "cDNA_Seq" ??? ???
Subsequence "cDNA_Seq" 200 800
> exon 200 300 "" Location "200..300"
> exon 450 800 "" Location "450..800"
> Intron 301 449 "" Location "301..449"
>> Sequence : "cDNA_Seq"
> DNA "cDNA_Seq" 500
Source "Genomic_Seq"
Source_Exon 1 101
Source_Exon 251 601
The Source_Exon tags will define the association with the Source
sequence. If the two sequences do not match you will see a warning on
the sequence display. I have not tried this but I think it should
work.
Mike
>>>> The 500 bp of the cDNA overlap the genomic DNA from
> 100-300 and from 450-750 (intro-exon junction in the
> cDNA is between nucs 200 and 201)
>> I appreciate any suggestions ...
> Thanks
> -Doug
>> Doug Smith, Professor of Biology .... dsmith at ucsd.edu> Department of Biology, 0116; UCSD; La Jolla CA 92093
> phone: 858-534-2620 FAX: 858-534-7108
> NOTE the area code: 619 changed to 858 in June, 1999!
>http://www-biology.ucsd.edu/others/dsmith/dnasys.html>>>
