Could someone suggest how to handle the following in a .ace file?
I have a cDNA that overlaps an exon-intron boundary of a genomic
sequence, and wish to encode the cDNA as a Subsequence of the
genomic.
How do I handle the cDNA coordinates to get proper graphic display
of the sequences?
Example:
Sequence : "Genomic_Seq"
DNA "Genomic_Seq" 1000
Subsequence "cDNA_Seq" ??? ???
exon 200 300 "" Location "200..300"
exon 450 800 "" Location "450..800"
Intron 301 449 "" Location "301..449"
Sequence : "cDNA_Seq"
DNA "cDNA_Seq" 500
The 500 bp of the cDNA overlap the genomic DNA from
100-300 and from 450-750 (intro-exon junction in the
cDNA is between nucs 200 and 201)
I appreciate any suggestions ...
Thanks
-Doug
Doug Smith, Professor of Biology .... dsmith at ucsd.edu
Department of Biology, 0116; UCSD; La Jolla CA 92093
phone: 858-534-2620 FAX: 858-534-7108
NOTE the area code: 619 changed to 858 in June, 1999!
http://www-biology.ucsd.edu/others/dsmith/dnasys.html
