IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Yeast] 5-FOA

kgeiler77 via yeast%40net.bio.net (by kgeiler from fas.harvard.edu)
Mon Jun 27 12:53:26 EST 2011


I was wondering what false positive rate to expect when plating yeast on
5-FOA. Are these false positives due to Ura+ colonies that manage to live on
this media for stochastic reasons (and can be screened for by replica
plating onto 5-FOA a second time), or do they represent mutations that allow
survival of Ura+ colonies on 5-FOA?

Any help, even general comments on false positives and 5-FOA, would be
greatly appreciated.

View this message in context: http://old.nabble.com/5-FOA-tp31939996p31939996.html
Sent from the Bio.net - Yeast mailing list archive at Nabble.com.

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net