Hi, there,
Does anybody know the casue of a much more slower growth rate of transformans? we introduced a 5kb heterologous ABC transporter gene in to Saccharomyces cerevisiae single mutant for a complementation experiment( integration vector, URA3 selection), the transformants did show up on selective medium but stay as a very little tiny colonies even after incubation for up to one week. any trouble shooting tips on this issue?
Thanks in advance.
Ye
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