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[Yeast] RE: Yeast Digest, Vol 45, Issue 2

Shaoyu Wang via yeast%40net.bio.net (by S.Wang from uws.edu.au)
Thu Feb 5 14:09:39 EST 2009


Hello, Everyone,
 
FCY2 gene is an important purine-cytosine permease. However, there two more putative purine-cytosine permeases encoded by FCY21 and FCY22. But there is lack of information on exact substrates and regulation of their activity etc. Only information I get is:  Fcy21p and Fcy22p are very similar to Fcy2p but can not substitute its function.
 
I wonder if anyone have more information on FCY21 and FCY22 and their protein product functions. Any pointers are appreciated.
 
Thank you
 
Shaun

________________________________

From: yeast-bounces from oat.bio.indiana.edu on behalf of yeast-request from oat.bio.indiana.edu
Sent: Fri 6/02/2009 4:06 AM
To: yeast from magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 45, Issue 2



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Today's Topics:

   1. RE: Yeast Digest, Vol 45, Issue 1 (Andrew Carter)


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Message: 1
Date: Wed, 4 Feb 2009 09:27:26 -0800
From: "Andrew Carter" <cartera from cmp.ucsf.edu>
Subject: [Yeast] RE: Yeast Digest, Vol 45, Issue 1
To: <yeast from oat.bio.indiana.edu>, <yeast from magpie.bio.indiana.edu>
Message-ID: <000001c986ed$daa298f0$8fe7cad0$@ucsf.edu>
Content-Type: text/plain;       charset="us-ascii"

Hi Thomas
Glucose represses the Gal promoter whereas Raffinose doesn't.  If you try
growing up yeast in YPD (2% glucose) and then add galactose you will get no
(in my experience) induction.  If you grow up in YP raffinose and add
galactose the promoter will get turned on immediately.

It turns out however that you can first grow in glucose and then add
galactose.  You just have to make sure that you have used up all your
glucose.  I have been playing with this quite a lot recently (in a large
fermentor) and it works well.  The key for me was using 2X YP and 1% glucose
and waiting until growth plateaus.  Then add in 2% galactose (0.5% and 1%
also work) and induction turned on just fine.  I was using induction from a
gal promoter inserted in the genome and haven't tried it with a plasmid
based system, but my guess is it should work.  The real advantage is that
Raffinose gets really expensive if you want to scale up... 

Not sure why growth is slower in galactose than glucose but I see it alot
(final OD is usually lower as well).
Best wishes
Andrew






-----Original Message-----
From: yeast-bounces from oat.bio.indiana.edu
[mailto:yeast-bounces from oat.bio.indiana.edu] On Behalf Of
yeast-request from oat.bio.indiana.edu
Sent: Wednesday, February 04, 2009 9:06 AM
To: yeast from magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 45, Issue 1

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Today's Topics:

   1. raffinose metabolism (Thomas, Arun)


----------------------------------------------------------------------

Message: 1
Date: Wed, 4 Feb 2009 11:36:10 +0100
From: "Thomas, Arun" <Arun.Thomas from Biologie.Uni-Osnabrueck.DE>
Subject: [Yeast] raffinose metabolism
To: <yeast from magpie.bio.indiana.edu>
Message-ID:
       
<F69C1DF9630D414E9411B4CB41240ABD0B12E6 from XCH.biologie.Uni-Osnabrueck.DE>
       
Content-Type: text/plain;       charset="iso-8859-1"

Hi everyone,

To induce a gene under the GAL promoter from a 2micron plasmid, why is it
preferred to grow the cells in raffinose/glucose initially followed by
induction with galactose?

If cells grow slowly in galactose, why is it so?

Thank you,
Thomas



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