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[Yeast] raffinose metabolism

Thomas, Arun via yeast%40net.bio.net (by Arun.Thomas from Biologie.Uni-Osnabrueck.DE)
Wed Feb 4 05:36:10 EST 2009


Hi everyone, 

To induce a gene under the GAL promoter from a 2micron plasmid, why is it preferred to grow the cells in raffinose/glucose initially followed by induction with galactose?

If cells grow slowly in galactose, why is it so? 

Thank you,
Thomas



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