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[Yeast] Expression of membrane proteins in Saccharomyces Cervisae

jkellosa from mappi.helsinki.fi via yeast%40net.bio.net (by jkellosa from mappi.helsinki.fi)
Mon Sep 15 15:58:35 EST 2008

Dear all,

I have noticed that in some cases when S. cerevisae is grown in shaker  
flasks in order to express membrane proteins the shaking is done at  
quite slow speed (130-160 rpm). Has anyone any kind of clue why it is  
so ?

Could it have something to do with inhibiting the formation of foam ?  
If so does anyone know if antifoaming agents are detrimental for  
membrane protein production.

Thanks already beforehand.


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