Dear all,
I have noticed that in some cases when S. cerevisae is grown in shaker
flasks in order to express membrane proteins the shaking is done at
quite slow speed (130-160 rpm). Has anyone any kind of clue why it is
so ?
Could it have something to do with inhibiting the formation of foam ?
If so does anyone know if antifoaming agents are detrimental for
membrane protein production.
Thanks already beforehand.
-Juho
