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[Yeast] Acid-washed glass Beads (Cinzia Pagliuca)

lautys via yeast%40net.bio.net (by lautys from gmail.com)
Tue May 27 21:52:15 EST 2008


Dear Cinzia Pagliuca,

Autoclave and acid-wash all the glass beads again, it should be no problem
to reuse it.

1. autoclave (killing)
2. acid-wash (to dissolve and hydrolyze any contaminant, standard protocols
can be found easily online)

But I would rather buy new bottle, since it's too hassle to clean up those
beads.

regards,
Lau



> ------------------------------
>
> Message: 3
> Date: Tue, 27 May 2008 09:18:25 -0700 (PDT)
> From: Cinzia Pagliuca <cinthiait from yahoo.com>
> Subject: [Yeast] Acid-washed glass Beads
> To: yeast from oat.bio.indiana.edu
> Message-ID: <329149.46166.qm from web51105.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dear all,
>
> I' m triyng to break the yeast cells with a Bead-Beater. Since I use
> 100-200 ml of Glass Beads (sigma, acid washed Beads), I would like to re-use
> them. It is possible? Can you suggest how to do it?
>
> Many thanks in advice,
> Cinzia
>
>
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