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[Yeast] Re: Yeast Digest, Vol 36, Issue 7

Jim Young via yeast%40net.bio.net (by jyyoungjimmy from gmail.com)
Sun May 18 20:41:20 EST 2008


Hi,

If you cannot solve the problem finally, a commercial company may helpful to
you.
http://www.genscript.com/custom_protein_purification_characterization.html


On Thu, May 15, 2008 at 1:07 AM, <yeast-request from oat.bio.indiana.edu> wrote:

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>   1. (no subject) (jkellosa from mappi.helsinki.fi)
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> Message: 1
> Date: Wed, 14 May 2008 14:10:19 +0300
> From: jkellosa from mappi.helsinki.fi
> Subject: [Yeast] (no subject)
> To: yeast from magpie.bio.indiana.edu
> Message-ID: <1210763419.482ac89b7d908 from webmail.helsinki.fi>
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> Dear all,
>
> A lab technician who I work with got both pure protein and wanted protein
> with two contaminating bands, both of which molecular weight is higher than
> the molecular weight of the wanted protein, from different batches of
> protein purification. The molecular weights of the contaminating bands are
> not high enough to account for different oligomerization states of the
> wanted protein.
>
> The two contaminating protein bands hang on really tightly with the wanted
> protein and we have not been able to get rid of them.
>
> I dont know what is the difference between purifications which produce pure
> protein and the purifications which don't, but I suspect that at least
> there has been differences at how long the cells have been growing since
> they were harvested.
>
> This leads me to my question that I want to ask from you:
>
> Have you ever had problems with contaminating proteins (stable
> proteins/chaperons hanging along, modifications of the wanted protein ?)
> when S.cerevisae cells have been in a certain growth phase ?
>
> -Juho Kellosalo
>
>
>
>
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> End of Yeast Digest, Vol 36, Issue 7
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