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[Yeast] S.pombe pREP series plasmids question...

Valerie Wood via yeast%40net.bio.net (by val from sanger.ac.uk)
Fri Jun 20 03:12:23 EST 2008

Hello Jonathan,

I have prl10 mapped to the opposite strand to nmt1.

However, if you look at the high resolution transcript map, it does not 
appear to be detectable.

Nothing is published about prl10.
Somebody else may be able to comment further.


Jonathan Jacobs wrote:
> Hello! this is my first post to this listserv, so please pardon me if 
> I am a bit overly verbose...
> I was updating my sequence files & vector maps recently for various 
> plasmids and I noticed something that might be of  interest to anyone 
> using the 'pREP' series vectors in S.pombe.
> Watanabe T et.al. (2002) <http://www.ncbi.nlm.nih.gov/pubmed/12597277> 
> published a report that indicated there were a number of poly(A) 
> bearing RNAs without long open reading frames expressed in S.pombe (so 
> called 'prl' genes). One of them, prl10 (Genbank AB084822 
> <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=26006032>), 
> is a 692-bp transcript that could produce a small 40 a.a. protein. 
> What was interesting to me was that prl10 sits immediately upstream of 
> nmt1. Since the nmt1 promoter region is used in the  pREP series 
> plasmids, thus so is the entire prl10 gene. Whether or not the prl10 
> transcript is expressed from pREP vectors is not known (to me).
> So I was curious: has anyone else noticed this before? And is it known 
> whether or not pREP series plasmids express the prl10 transcript? And 
> finally, is it known if there is a phenotype associated with a prl10 
> deletion strain? Does anyone have a hint as to what the function of 
> this RNA may be?
> Jonathan Jacobs, Ph.D.
> lab: (301) 402-1155
> cell: (240) 447-4039
> http://www.linkedin.com/in/jonathanjacobs
> http://www.citeulike.org/user/jonathanjacobs
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Valerie Wood			 Tel: 01223 496909
S. pombe Genome Project		 Fax: 01223 494919 		       
Wellcome Trust Sanger Institute	 email: val from sanger.ac.uk
Wellcome Trust Genome Campus	 http://www.genedb.org/genedb/pombe 
Hinxton, Cambridge, CB10 1HH	 http://www.sanger.ac.uk/Projects/S_pombe

 The Wellcome Trust Sanger Institute is operated by Genome Research 
 Limited, a charity registered in England with number 1021457 and a 
 company registered in England with number 2742969, whose registered 
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