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[Yeast] cell cycle and temp (Hannah R Silver)

Dirk Müller via yeast%40net.bio.net (by dirk.mueller from inf.ethz.ch)
Thu Jun 5 13:21:53 EST 2008

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> Today's Topics:
>    1. cell cycle and temp (Hannah R Silver)
>    2. vitamins (Behzadnia, Dr. Nastaran)
> ----------------------------------------------------------------------
> Message: 1
> Date: Wed, 4 Jun 2008 14:36:49 -0400
> From: "Hannah R Silver" <hannah.silver from jefferson.edu>
> Subject: [Yeast] cell cycle and temp
> To: yeast from magpie.bio.indiana.edu
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> 	<40888dc80806041136p32b63ae9p43b1afd16825d92a from mail.gmail.com>
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> Hello. Does anyone know if the FACS profile for asynchronous cells looks
> different at 25C vs 36C? For example, do you see a higher accumulation in G1
> at 25 than you would at 36?
> Also has anyone else noticed the temporary arrest in G1 after galactose
> addition to asynch cells growing in YPR?
Hi Hannah,

while I have no personal experience with the temporary cell-cycle arrest 
on Galactose,
the increased G1 fraction at 25°C vs. 36°C is clearly expected.

As temperature decreases your cells grow more slowly.
This slower growth is mostly attributable to cells spending more time in G1.
Since at low growth rates daughter cells take much longer than mother 
cells to complete their cycles,
you will have an increased fraction of cells in G1 (mothers and 
daughters) and a higher fraction of 
daughter cells (many in G1) in the population. This is consistent with 
your FACS results.

There are several papers describing this effect, e.g.
Hartwell, L.H., and M.W. Unger (1977)  /J. Cell Biol./ *75*(2): 422-435.
Lord, P.G., and A.E. Wheals (1980).  /J. Bact./ *142*(3): 808-818. 




Dr. Dirk Mueller

Institute of Computational Science
Computational Systems Biology Group
ETH Zurich
Universitaetstr. 6, CAB J71.6
CH-8092 Zurich

Tel. ++41-(0)44-63-2-6434
Fax. ++41-(0)44-63-2-1374
Email: dirk.mueller from inf.ethz.ch
Web:   http://csb.inf.ethz.ch

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