We used N-tert-butyl-alpha-phenylnitrone(PBN) to quenching the ROS in
yeast. It works well.
Here is the reference:
Qun Ren et al., (2005) Mutation of the cohesin related gene PDS5 causes
cell death with predominant apoptotic features in Saccharomyces
cerevisiae during early meiosis Mutation Research 570:163-173
Let me know if you need a copy of the reprint.
Zhaojie Zhang, Ph.D.
Department of Zoology and Physiology
University of Wyoming
Laramie, WY 82071
zzhang from uwyo.eduhttp://www.uwyo.edu/microscopy
From: Juxiang Cao [mailto:CAOJUXI from auburn.edu]
Sent: Wednesday, September 12, 2007 8:20 PM
To: yeast from magpie.bio.indiana.edu
Subject: [Yeast] ROS scavenger
Does anybody know any ROS quenching reagent we can use to add to yeast
medium so that cells will be protected from the ROS damage under stress
conditions? if you can help, please also send the references, thanks a
Yeast mailing list
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