PLEASE, I NEED CONVERT GLYCERIN TO METANOL; IF ANYWHERE HAVE A WAY PLEASE
C. I. Hassun
2007/9/5, Wang, Xin-Xiang <WangXX from medimmune.com>:
>> Hello Kerry,
>> I used to induce yeast for GFP expression, also using Gal promoter. I
> noticed the trace amount of repressing medium (raffinose in your case or
> glucose in my case) was essential for GFP induction. If I washed the trace
> amount of repressing medium away with induction medium, GFP was not induced
> in even a week! But with the trace amount of glucose medium in, induction
> took only 15 hours (not test shorter time).
>> Did you wash away the raffinose medium before induction or not? Good luck!
>> From: yeast-bounces from oat.bio.indiana.edu on behalf of
>yeast-request from oat.bio.indiana.edu> Sent: Wed 9/5/2007 1:02 PM
> To: yeast from magpie.bio.indiana.edu> Subject: Yeast Digest, Vol 28, Issue 2
>>>> Send Yeast mailing list submissions to
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>>> Today's Topics
>> 1. Slow galactose induction (Kerry Geiler)
> 2. RE: Slow galactose induction (Z.J. Zhang)
>> Message: 1
> Date: Tue, 4 Sep 2007 13:54:57 -0400
> From: "Kerry Geiler" <kgeiler from gmail.com>
> Subject: [Yeast] Slow galactose induction
> To: yeast from magpie.bio.indiana.edu> Message-ID:
> <3fa3f1ca0709041054o692505aek8f75bbd8b3d1d072 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>> I am looking for suggestions as to why my galactose induction process
> takes 6 hours. I am expressing CFP under a gal promoter in media with
> no glucose and 2% galactose. I am getting fluorescence, but it takes
> 6 hours to see it. Any ideas why induction could be taking so long?
>> I grow the cells up in media containing no glucose (2%raffinose)
> overnight. Then I induce 150uL of this culture with 2% galactose and
> measure fluorescence every 15 minutes. I do not see fluorescence
> distinguishable from background until about 6 hours later.
>> Thanks very much for any suggestions! Please reply to kgeiler from gmail.com.> Thanks,
>> Message: 2
> Date: Tue, 4 Sep 2007 15:27:43 -0600
> From: "Z.J. Zhang" <ZZhang from uwyo.edu>
> Subject: RE: [Yeast] Slow galactose induction
> To: "Kerry Geiler" <kgeiler from gmail.com>, <yeast from magpie.bio.indiana.edu>
> <C9C1AF307F12AF4087DEF87CB0E6A4AD01A3881F from TELEGRAPH5.uwyo.edu>
> Content-Type: text/plain; charset="US-ASCII"
>> One possibility is the slow maturation of your CFP protein, rather than
> slow induction.
>> Depending on the version of the CFP, and where the CFP is tagged, it
> could take hour(s) to mature.
>> Zhaojie Zhang, Ph.D.
> Director, Microscopy Core Facility
> Department of Zoology and Physiology
> University of Wyoming
> Laramie, WY 82071
> TEL: 307-766-3038
> FAX: 307-766-5625
>zzhang from uwyo.edu>http://www.uwyo.edu/microscopy>>>> _______________________________________________
> Yeast mailing list
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