Hello Jean Nicolas,
In fission yeast, there is no variation in the genetic background, like in
budding yeast, and I'd say changing the strains is not going to make much
difference. I don't know what FY261 and FY254 are (probably the stock
numbers of some guy whose initials are FY). Just use any WT as a control, as
long as the cells look happy, you're good. Only, if you want to compare
various mutants, try to use strains that have the same auxotrophy markers.
I was told, and it works, to prepare the glucose-free plates by adding 0.1%
glucose anyway. The cells need it to initiate there growth, they use it all,
and then start using the other source of carbon. It nevertheless takes ages
to see the first colonies. I have never noticed any variation in colony size
and cannot help you on this matter.
I hope this helps.
Very good luck,
Isabelle
-----Original Message-----
From: yeast-bounces from oat.bio.indiana.edu
[mailto:yeast-bounces from oat.bio.indiana.edu] On Behalf Of
yeast-request from oat.bio.indiana.edu
Sent: Thursday, 15 November 2007 6:05 a.m.
To: yeast from magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 30, Issue 1
Send Yeast mailing list submissions to
yeast from net.bio.net
To subscribe or unsubscribe via the World Wide Web, visit
http://www.bio.net/biomail/listinfo/yeast
or, via email, send a message with subject or body 'help' to
yeast-request from net.bio.net
You can reach the person managing the list at
yeast-owner from net.bio.net
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Yeast digest..."
Today's Topics:
1. pombe respiration (Jean-Nicolas Jasmin)
----------------------------------------------------------------------
Message: 1
Date: Wed, 14 Nov 2007 02:38:11 -0800
From: Jean-Nicolas Jasmin <jejasmin from ucsd.edu>
Subject: [Yeast] pombe respiration
To: yeast from magpie.bio.indiana.edu
Message-ID: <200711132235.lADMZu5c003112 from biomail.ucsd.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hi,
I am working with Sz pombe strains FY261 and FY254 and I find it
difficult to grow them on a non-fermentable substrate. I have tried
solid media with yeast extract (1 to 5%) and glycerol (1 to 3%) and
ethanol (1 to 3%), plus amino acids. On 5% YE and 2% glycerol and
ethanol it takes about 2 weeks to get large colonies and the colonies
show very high variation in size.
Is this normal for this genotype?
Which pombe strain should I use to obtain better growth on
non-fermentable substrates?
Many thanks,
Jean-Nicolas
EBE, Biological Sciences, UCSD
------------------------------
_______________________________________________
Yeast mailing list
Yeast from net.bio.nethttp://www.bio.net/biomail/listinfo/yeast
End of Yeast Digest, Vol 30, Issue 1
************************************
