Hi,
I am working with Sz pombe strains FY261 and FY254 and I find it
difficult to grow them on a non-fermentable substrate. I have tried
solid media with yeast extract (1 to 5%) and glycerol (1 to 3%) and
ethanol (1 to 3%), plus amino acids. On 5% YE and 2% glycerol and
ethanol it takes about 2 weeks to get large colonies and the colonies
show very high variation in size.
Is this normal for this genotype?
Which pombe strain should I use to obtain better growth on
non-fermentable substrates?
Many thanks,
Jean-Nicolas
EBE, Biological Sciences, UCSD
