Dear all,
Has anyone done RT-PCR from spheroplast? I mean using spheroplast as template without extracting total RNA. Originally I just used water to lyze spheroplast but all of a sudden my RTPCR has stopped working. This is telling me I have been working on the limit. Any idea how to eliminate the genomic DNA/cellular protein contaminant while keeping the integrity of mRNA? Thanks!
BTW, RT=reverse transcription.
Regards,
Xinxiang
