Andreea,
Sometimes you can increase sporulation efficiency by first streaking out
your diploid strain onto presporulation medium before putting it on KAc
plates. You can either order a mix or make it yourself - we use 4.8g yeast
extract, 1.8g peptone, 60g d-glucose, and 9 g agar for 600ml.
Regards,
Samantha Herbst
--
Samantha Herbst
Craig Lab
433 Babcock Dr, room 419
Madison, WI 53706
saherbst from biochem.wisc.edu
> ------------------------------
>> Message: 3
> Date: Tue, 15 May 2007 16:25:17 +0200
> From: "Daraba Andreea" <Andreea from nucleus.szbk.u-szeged.hu>
> Subject: [Yeast] sporulation
> To: yeast from magpie.bio.indiana.edu> Message-ID: <4649DEED.20718.3B91B1 from nucleus.szbk.u-szeged.hu>
> Content-Type: text/plain; charset=US-ASCII
>> Dear Everyone
> Can anyone tell me how to increase sporulation efficiency in yeast?
> I am suppose to create double deletion mutants using tetrade analisys but
> unfortunately the sporulation efficiency is quite low and I am having
> troubles finding
> tetrades.
>> Thank you,
> Andreea
>> Andreea Daraba
> DNA Repair Research Group
> Institute of Genetics
> Biological Research Center
> Hungarian Academy of Science
>>
