Dear Daraba
On your question about getting yeast to sporulate more...
I frequently get Sacharomyces spp. yeast to sporulate at a high rate
by reducing availabilty of one of the essential growth parameters
(like carbon source, pH etc.) needed by yeast. For example if you
slowly decrease the pH of the growth media that you grow the yeast in
they will sporulate.
Hope this helps
regards
Pieter Swanepoel
Brewing Support Scientist
Cara Technology Limited
pieter.swanepoel from cara-online.com
www.cara-online.com
Tel +44 1372 822 233
Fax +44 1372 821 599
Leatherhead Enterprise Centre
Randalls Road
Leatherhead
Surrey
KT22 7RY
UK.
Registered in England and Wales, Company number: 2242098
VAT number: GB 492 3199 23
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Quoting yeast-request from oat.bio.indiana.edu:
> Send Yeast mailing list submissions to
>yeast from net.bio.net>> To subscribe or unsubscribe via the World Wide Web, visit
>http://www.bio.net/biomail/listinfo/yeast> or, via email, send a message with subject or body 'help' to
>yeast-request from net.bio.net>> You can reach the person managing the list at
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> than "Re: Contents of Yeast digest..."
>>> Today's Topics:
>> 1. 3-at (luca.lotito3)
> 2. yeast DNA library (Ahmet Koc)
> 3. sporulation (Daraba Andreea)
> 4. Re: 3-at (John Bida)
>>> ----------------------------------------------------------------------
>> Message: 1
> Date: Mon, 14 May 2007 11:01:20 +0200
> From: "luca.lotito3" <luca.lotito3 from unibo.it>
> Subject: [Yeast] 3-at
> To: <yeast from magpie.bio.indiana.edu>
> Message-ID: <000601c79606$706bc670$c3a3cc89 from step>
> Content-Type: text/plain; charset="us-ascii"
>>>> Hi,
>> i want to use 3-at to analyzed silencing of his5 gene of s.pombe cloned at
> telomere of chromosome XIV of. S. cerevisiae.
>> Someone have a protocols to use that drug? I have test it but the yeast did
> not die at 200mM of 3-at. I test the drug also in other strains with wt his3
> gene, but the results are the same: the yeast did not die.
>> Someone help me?
>> thanks
>> ++++++++++++++++++++++++++++++++++++++++++
>> Luca Lotito, PhD
>> Department of Biochemistry, University of Bologna
>> via Irnerio 48, 40126 Bologna, Italy
>> Phone: +39 051 2094282
>> Fax: +39 051 2094283
>> <http://www.biotecnologie.unibo.it/capranicolab/index.php>
>http://www.biotecnologie.unibo.it/capranicolab/index.php>>>> -------------- next part --------------
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>http://www.bio.net/bionet/mm/yeast/attachments/20070514/11de4124/attachment-0001.html>> ------------------------------
>> Message: 2
> Date: Tue, 15 May 2007 11:05:47 +0300
> From: "Ahmet Koc" <ahmetkoc from iyte.edu.tr>
> Subject: [Yeast] yeast DNA library
> To: <yeast from magpie.bio.indiana.edu>
> Message-ID: <000701c796c7$db8ba990$3b0c0a0a from koc>
> Content-Type: text/plain; charset="us-ascii"
>> Hi all,
> I would like to obtain a yeast (S.c.) genomic DNA library on a high copy
> yeast expression vector. Any markers OK
> Does anyone know if I can purchase it.
>> Ahmet Koc, Ph.D.
> Izmir Institute of Technology
> Department of Biology
> 35430,Urla, Izmir, Turkey
> Tel: +90-232-750 7544
> Fax:+90-232-750 7509
>ahmetkoc from iyte.edu.tr>>>> -------------- next part --------------
> An HTML attachment was scrubbed...
> URL:
>http://www.bio.net/bionet/mm/yeast/attachments/20070515/73829020/attachment-0001.html>> ------------------------------
>> Message: 3
> Date: Tue, 15 May 2007 16:25:17 +0200
> From: "Daraba Andreea" <Andreea from nucleus.szbk.u-szeged.hu>
> Subject: [Yeast] sporulation
> To: yeast from magpie.bio.indiana.edu> Message-ID: <4649DEED.20718.3B91B1 from nucleus.szbk.u-szeged.hu>
> Content-Type: text/plain; charset=US-ASCII
>> Dear Everyone
> Can anyone tell me how to increase sporulation efficiency in yeast?
> I am suppose to create double deletion mutants using tetrade analisys but
> unfortunately the sporulation efficiency is quite low and I am
> having troubles finding
> tetrades.
>> Thank you,
> Andreea
>> Andreea Daraba
> DNA Repair Research Group
> Institute of Genetics
> Biological Research Center
> Hungarian Academy of Science
>>>> ------------------------------
>> Message: 4
> Date: Tue, 15 May 2007 10:01:48 -0500
> From: John Bida <Bida.John from mayo.edu>
> Subject: Re: [Yeast] 3-at
> To: "luca.lotito3" <luca.lotito3 from unibo.it>
> Cc: yeast from magpie.bio.indiana.edu> Message-ID: <D7D4E8FE-998C-4C88-B466-0D447E8D3662 from mayo.edu>
> Content-Type: text/plain; charset="us-ascii"
>> Are you using -HIS drop out media with 3-AT?
>> ~Jp Bida
>>>>> On May 14, 2007, at 4:01 AM, luca.lotito3 wrote:
>>>>> Hi,
>>>> i want to use 3-at to analyzed silencing of his5 gene of s.pombe
>> cloned at telomere of chromosome XIV of. S. cerevisiae.
>>>> Someone have a protocols to use that drug? I have test it but the
>> yeast did not die at 200mM of 3-at. I test the drug also in other
>> strains with wt his3 gene, but the results are the same: the yeast
>> did not die.
>>>> Someone help me?
>>>> thanks
>>>> ++++++++++++++++++++++++++++++++++++++++++
>>>> Luca Lotito, PhD
>>>> Department of Biochemistry, University of Bologna
>>>> via Irnerio 48, 40126 Bologna, Italy
>>>> Phone: +39 051 2094282
>>>> Fax: +39 051 2094283
>>>>http://www.biotecnologie.unibo.it/capranicolab/index.php>>>>>> _______________________________________________
>> Yeast mailing list
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>Yeast from net.bio.net>http://www.bio.net/biomail/listinfo/yeast>> End of Yeast Digest, Vol 24, Issue 5
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