Are you using -HIS drop out media with 3-AT?
~Jp Bida
On May 14, 2007, at 4:01 AM, luca.lotito3 wrote:
>> Hi,
>> i want to use 3-at to analyzed silencing of his5 gene of s.pombe
> cloned at telomere of chromosome XIV of. S. cerevisiae.
>> Someone have a protocols to use that drug? I have test it but the
> yeast did not die at 200mM of 3-at. I test the drug also in other
> strains with wt his3 gene, but the results are the same: the yeast
> did not die.
>> Someone help me?
>> thanks
>> ++++++++++++++++++++++++++++++++++++++++++
>> Luca Lotito, PhD
>> Department of Biochemistry, University of Bologna
>> via Irnerio 48, 40126 Bologna, Italy
>> Phone: +39 051 2094282
>> Fax: +39 051 2094283
>>http://www.biotecnologie.unibo.it/capranicolab/index.php>>> _______________________________________________
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>Yeast from net.bio.net>http://www.bio.net/biomail/listinfo/yeast
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