You may hear from someone who has used a particular gene for this
purpose, but if not, you can use SGD's Expression Connection
to investigate this. Using Search III, which allows you to find genes
whose expression changes by the manner you specify, to find genes
whose expression doesn't change significantly in the dataset
"Expression in response to environmental changes" (A.P. Gasch et
al.), should reveal some candidates. One of the conditions tested in
this study was hyperosmotic stress.
Maria C. Costanzo, Ph.D.
Senior Scientific Curator, Saccharomyces Genome Database
and Candida Genome Database
Department of Genetics
Stanford University School of Medicine
Stanford, CA 94305-5120
http://www.yeastgenome.org/http://www.candidagenome.org/maria from genome.stanford.edu
On Mar 27, 2007, at 2:22 AM, Lisa Ooms wrote:
> I'm trying to find a reference gene for RT-PCR on S. cerevisiae
> following hyperosmotic stress. Can anyone suggest a gene that does not
> alter in response to NaCl stress?
>> Thanks for your help.
>> Lisa Ooms
> Monash University
> Yeast mailing list
>Yeast from net.bio.net>http://www.bio.net/biomail/listinfo/yeast
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