Hi, Anna,
I truly don't see why it would be a problem. Actually, those cultures will
be growing very good and the cells will be quite robust. What I'd do is to
take the cells that you need for the transformation and place the rest in
the incubator while you are transforming, and, by the time you finish the
transformation, your cells will be probably nicely saturated and ready to
freeze (of course, with glycerol).
-----Original Message-----
From: yeast-bounces At oat.bio.indiana.edu
[mailto:yeast-bounces At oat.bio.indiana.edu] On Behalf Of
yeast-request At oat.bio.indiana.edu
Sent: Friday, January 19, 2007 9:04 AM
To: yeast At magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 20, Issue 4
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Today's Topics:
1. succinic semialdehyde determination (Juxiang Cao)
2. Glycerol stocks from cultures in 2xYPAD (Anna Heidenblut)
3. pombe ura4 library (Rich Maraia)
----------------------------------------------------------------------
Message: 1
Date: Thu, 18 Jan 2007 10:17:02 -0600
From: "Juxiang Cao" <caojuxi At auburn.edu>
Subject: [Yeast] succinic semialdehyde determination
To: <Yeast At magpie.bio.indiana.edu>
Message-ID: <45AF491E02000007002C18B9 At TMIA1.AUBURN.EDU>
Content-Type: text/plain; charset=US-ASCII
Hi,
I would like to determine the succinic semialdehyde level from heat
stressed yeast cells by enzymatic assay, could I simply boil the cells
to prepare the crude cell extract? How is the stability of SSA?
Best regards
Juxiang Cao
------------------------------
Message: 2
Date: Fri, 19 Jan 2007 10:43:50 +0000
From: Anna Heidenblut <anna.heidenblut At gmx.de>
Subject: [Yeast] Glycerol stocks from cultures in 2xYPAD
To: yeast At magpie.bio.indiana.edu
Message-ID: <45B0A0E6.2090908 At gmx.de>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear all,
another elementary question:
Can it cause any problems when I prepare glycerol stocks from cultures
that were grown in double strength YPAD (YPD with additional adenin
added)? I use this 2xYPAD to grow pre-cultures for yeast transformation
and it would be very convenient to prepare glycerol stocks from the
pre-cultures immediately rather than setting up a separate culture in
YPD just for the preparation of glycerol stocks.
Any comments on that question are highly appreciated.
Anna
--
Dr. Anna Heidenblut (PhD)
Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419
anna.heidenblut At cancer.org.uk
------------------------------
Message: 3
Date: Fri, 19 Jan 2007 09:28:03 -0500
From: Rich Maraia <maraiar At exchange.nih.gov>
Subject: [Yeast] pombe ura4 library
To: yeast At magpie.bio.indiana.edu
Message-ID: <p06230903c1d685c345ea@[137.187.221.10]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Does anyone have or know of a S. pombe genomic library on a
ura4+-containing plasmid?
--
Richard J. Maraia, M.D.
Captain, US Public Health Service, Commissioned Corps
Chief, Section on Molecular and Cell Biology
Genomics of Differentiation
National Institute of Child Health and Human Development
National institutes of Health
31 Center Drive,
Bld 31, Rm 2A25 MSC-2426
Bethesda, MD 20892-2426
Phone: 301 402-3567
Fax: 301 480-6863
http://eclipse.nichd.nih.gov/nichd/Maraia/Maraialabpage.html
------------------------------
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