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[Yeast] alpha factor arresting in bar1 strains

Hannah R Silver via yeast%40net.bio.net (by hannah.silver from jefferson.edu)
Fri Dec 21 17:08:51 EST 2007

alpha factor arresting in bar1 strains is a fairly common practice so
i think that others must see this effect also. the first time i did
the arrest the cells did not release synchronously and also did not
release until 45-60min after washout (at 60min many cells were small
budded but others we still shmooing). the next time i used a protease,
pepsin, i washed 1x in YPR, 2x pepsin in YPR, 1x in YPR then released
into YPR. still the cells did not release until after 45-60min but at
least this time they were more synchronous but not quite perfect. ive
ordered the pronase E protease that the koshland webpage recommends
but im not sure if the delayed in the release of the cells from G1 is
common for others. can anyone advise here?

HR Silver
PhD Candidate
Department of Biochemistry and Molecular Biology
Thomas Jefferson University
233 South 10th Street, BLSB 231
Philadelphia, Pennsylvania 19107

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