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[Yeast] Sacc. Kluyveri

paul m via yeast%40net.bio.net (by pmmagic from gmail.com)
Tue Dec 11 15:04:33 EST 2007


A book that might be useful is:

Wolf, Bruenig, Barth (Eds.). 2003. Non-convential yeasts in genetics,
biochemistry, and biotechnology. Springer.

There's nothing specifically on S. kluyveri but it does have a couple of
protocols for doing gene disruptions in K. lactis which is known to exhibit
low rates of homologous recombination.

Cheers,
Paul Magwene


On Dec 10, 2007 11:09 AM, Christian Landry <clandry from post.harvard.edu> wrote:

> Hello,
> Does anyone know if it is feasible to do homologous recombination (with
> PCR products) of Saccharomyces kluyveri? Do you know any reference?
> Finally,  do you know who would have haploid strains (a and alpha) for
> this species with selectable markers?
>
> Thanks a lot,
>
> Christian
>
>
>
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