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[Yeast] Mating type switching in S. cerevisiae Katrin Hartwich

John McCusker via yeast%40net.bio.net (by mccus001 from mc.duke.edu)
Mon Dec 3 11:23:37 EST 2007


My former advisor, Jim Haber, provided SGD with 
some text that explains the problem: see 
then go to "summary paragraph" 

Basically, MATa in the S288c background (BY4141 
is S288c background) has a single base pair 
polymorphism that greatly impairs (but does not 
completely prevent) MAT switching.


John McCusker

Hello to all,

I'm working with the S. cerevisiae strains from the EUROSCARF collection
(BY4741). All strains have the mating type a and I want to switch now the
mating type of some of them to alpha. I have a protocol and a plasmid for
mating type switching from a friend but it didn't work that well. Does
anybody have a good working protocol/plasmid for mating type switching for
Thank's a lot!

Best wishes,

Katrin Hartwich
European Neuroscience Institute Göttingen (ENI-G)
Molecular Neurogenetics

Grisebachstraße 5
37073 Göttingen

Phone: +49 (0)551-39-13972
Fax: +49 (0)551-39-12346
Email: khartwi from gwdg.de

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Dept. of Molecular Genetics & Microbiology, 3020
Duke University Medical Center
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e-mail: mccus001 from mc.duke.edu

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