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[Yeast] Mating type switching in S. cerevisiae Katrin Hartwich

John McCusker via yeast%40net.bio.net (by mccus001 from mc.duke.edu)
Mon Dec 3 11:23:37 EST 2007


Hello,

My former advisor, Jim Haber, provided SGD with 
some text that explains the problem: see 
  <http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat 
then go to "summary paragraph" 
 <http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph

Basically, MATa in the S288c background (BY4141 
is S288c background) has a single base pair 
polymorphism that greatly impairs (but does not 
completely prevent) MAT switching.

Regards,

John McCusker

*****************************************
Hello to all,

I'm working with the S. cerevisiae strains from the EUROSCARF collection
(BY4741). All strains have the mating type a and I want to switch now the
mating type of some of them to alpha. I have a protocol and a plasmid for
mating type switching from a friend but it didn't work that well. Does
anybody have a good working protocol/plasmid for mating type switching for
me?
Thank's a lot!

Best wishes,
Katrin



--
Katrin Hartwich
European Neuroscience Institute Göttingen (ENI-G)
Molecular Neurogenetics

Grisebachstraße 5
37073 Göttingen
Germany

Phone: +49 (0)551-39-13972
Fax: +49 (0)551-39-12346
Email: khartwi from gwdg.de

--
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John H. McCusker, Assoc. Prof.
Dept. of Molecular Genetics & Microbiology, 3020
Duke University Medical Center
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