Hello,
My former advisor, Jim Haber, provided SGD with
some text that explains the problem: see
<http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat
then go to "summary paragraph"
<http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph>http://db.yeastgenome.org/cgi-bin/locus.pl?locus=mat#summaryParagraph
Basically, MATa in the S288c background (BY4141
is S288c background) has a single base pair
polymorphism that greatly impairs (but does not
completely prevent) MAT switching.
Regards,
John McCusker
*****************************************
Hello to all,
I'm working with the S. cerevisiae strains from the EUROSCARF collection
(BY4741). All strains have the mating type a and I want to switch now the
mating type of some of them to alpha. I have a protocol and a plasmid for
mating type switching from a friend but it didn't work that well. Does
anybody have a good working protocol/plasmid for mating type switching for
me?
Thank's a lot!
Best wishes,
Katrin
--
Katrin Hartwich
European Neuroscience Institute Göttingen (ENI-G)
Molecular Neurogenetics
Grisebachstraße 5
37073 Göttingen
Germany
Phone: +49 (0)551-39-13972
Fax: +49 (0)551-39-12346
Email: khartwi from gwdg.de
--
*************************************************
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