i have integrated a SSN6 domain with ADH1 promoter and hCMV promoter into
the genome of yeast.For my further expts i need to chek their growth on
nonfermantable carbo source.but i have doneintegrative transformation at
least 4 times succesfully ,but then they dont grow on growth on
nonfermatable carbon source.
So my query is that ,Does the integration of SSN6 into the yeast genome
affect such phenotype that it renders no growth on nonfermatable carbon
source???(because SSN6 is a genral transcrition co repressor).
Thanks
You can reply me at vipulgreat At gmail.com
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