Hello,
I am a student working at the NIH/NICHD LMG. I have deleted a gene, Maf1,
in two S. pombe strains and am working at characterising the mutants. I
would like to grow my strains on a non-fermentable carbon source to
establish if there is a ts phenotype on non-fermentable carbon, as there is
with cerevisiae upon Maf1 deletion. I have gotten plates which are
essentially YES rich media, but contain 3% glycerol in place of glucose.
The parent strain grew extremely slowly, and upon looking at the cells under
a microscope, the parent strain exhibits a different morphology than what
I'm used to seeing with pombe. The cells are slightly larger, and are quite
round, not perfectly round, but more so than the S. pombe cells I looked at
which were growing on standard YES.
My questions are: Am I using a good media for this analysis, 3% glycerol
Rich Media with no glucose? Does anyone have experience with S. pombe
giving a different morphology under these conditions? Any assistance would
be greatly appreciated.
