Dear yeast users
I am trying to integrate an integrative plasmid into Gap1 locus from
Saccharomyces cerevisiae. For that propose I am using a non buffered
minimal media (0.17% YNB w/o Nitrogen, 0.1% proline, 20mg/L uracile
and 2% glucose) containing 10mM of D-Histine. However, I am getting a
huge number of colonies in my control plate (yeast transformed
without plasmid).
Does anyone have an idea how I can decrease this background ? Could
it be a pH problem, concentration of D-Histidine ?
Many thanks in advance for your recommendations.
Dominique
Dominique Loque
Carnegie Institution of Washington
Department of Plant Biology
260 Panama Street
Stanford, CA 94305
USA
Tel. 650 325 1521 ext 249
Fax 650 325 6857
Email: dloque at stanford.edu
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