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[Yeast] Integration in Gap1 locus / D-Histidine selection

Dominique Loque dloque at stanford.edu
Fri Jan 20 23:46:27 EST 2006

Dear yeast users

I am trying to integrate an integrative plasmid into Gap1 locus from 
Saccharomyces cerevisiae. For that propose I am using a non buffered 
minimal media (0.17% YNB w/o Nitrogen, 0.1% proline, 20mg/L uracile 
and 2% glucose) containing 10mM of D-Histine. However, I am getting a 
huge number of colonies in my control plate (yeast transformed 
without plasmid).
Does anyone have an idea how I can decrease this background ? Could 
it be a pH problem, concentration of D-Histidine ?

Many thanks in advance for your recommendations.


Dominique Loque

Carnegie Institution of Washington
Department of Plant Biology
260 Panama Street
Stanford, CA 94305

Tel. 650 325 1521 ext 249
Fax 650 325 6857
Email: dloque at stanford.edu
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