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[Yeast] Plasmid isolation and colony PCR

pooja at mbu.iisc.ernet.in pooja at mbu.iisc.ernet.in
Fri Apr 14 07:46:35 EST 2006


Hello everyone,
I wish to do a colony PCR with S.pombe. The PCR involves amplification of
a gene sequence(1600 bp) from a plasmid that has been transformed in
S.pombe. As i was goung through some references, i think i can directly
use the boiling of colony followed by PCR reaction instead of using the
zymolyase solution.
Please tell me whether i am correct.

Please also tell me where can i find a protocol for isolation of plasmid
DNA from S.pombe.

Thanks.

Regards,
Pooja



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