I wish to do a colony PCR with S.pombe. The PCR involves amplification of
a gene sequence(1600 bp) from a plasmid that has been transformed in
S.pombe. As i was goung through some references, i think i can directly
use the boiling of colony followed by PCR reaction instead of using the
Please tell me whether i am correct.
Please also tell me where can i find a protocol for isolation of plasmid
DNA from S.pombe.