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background growth / uracil marker

Bruno.Dombrecht at csiro.au Bruno.Dombrecht at csiro.au
Tue Oct 12 22:35:25 EST 2004


I've just started working on Y2H with Stratagene's Cytotrap system.
This system uses the cdc25H strain in combination with URA3 as
selection marker on the target-plasmid.  However, I seem to get quite
some background growth of cdc25H on SD lacking uracil.  Does anyone
have an explanation for this?
 
 
Cheers,
 
Bruno
 
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