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permeability

[ISO-8859-2] Olga Jedli=E8kov=E1 jedla at bf.jcu.cz
Fri May 7 07:47:49 EST 2004


Dear everyone

I am trying to measure the transport of radioactively labelled (3H)
cyclosporin (eleven aminoacids in cirle, 1200 Da) through the plasma
membrane of S. cerevisiae. Unexpectably I did not get any meaningful
values- it looks like cyclosporin does not enter the cells at all. I
use whole cells with cell walls (for to withstand the
filtration). What could be wrong?

Thanks very much

Olga Jedlickova

Faculty of biology
University of South Bohemia
Ceske Budejovice
Czech Republic

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